TABLE 4.

Results of phosphatase activity assays

Substrate	Sp act (μmol/min/mg) for:			Km (mM)
	PhoEa	Rv3214	Positive control
3-Phosphoglycerate	27	0.00
α-Naphthyl phosphate	21	0.00
p-Nitrophenyl phosphate	16	2.6 ± 0.03	0.12 ± 0.02	0.04
AMP	5	0.00
ADP		2.4 × 10−3 ± 6 × 10−4		0.25
2,3-Bisphosphoglycerate		0.00
Phosphoenolpyruvate		0.00
Phytic acid		5.8 × 10−2 ± 6 × 10−3		0.068
Coenzyme A		0.00
Sucrose-6-phosphate		1.5 × 10−2 ± 2 × 10−3		0.014
Fructose-6-phosphate		5.9 × 10−3 ± 6 × 10−4		0.3
Maltose-1-phosphate		0.00
Glycerol-1-phosphate		5.9 × 10−3 ± 4 × 10−4		0.16
Ribose-5-phosphate		0.00
Dihydroxyacetone phosphate		3.0 × 10−2 ± 3 × 10−3		1.62
Phosphatidic acid		0.00

^aSee reference 37.