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. 2006 Jun;188(11):3774–3784. doi: 10.1128/JB.00136-06

FIG. 5.

FIG. 5.

(A) Genetic organization of the sequenced 4,500 bp from plasmid pEctD16. (B) Generation of a construct carrying a mutated ectD gene. pRectD1 is a subclone of pEctD16 carrying the orf2-ectD-orf4 region. In pRectD4, the ectD gene was inactivated by the insertion of an Ω cassette, which carries resistance genes for streptomycin and spectinomycin, into its unique Bsp119I site (B).