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ectD is required for the main ectoine hydroxylase activity in C. salexigens. Cells of the C. salexigens strains CHR62 (ΔectABC) and CHR137 (ΔectABC ΔectD) were grown at 37°C in M63 minimal medium with 1.5 M NaCl in the presence of 1 mM ectoine (E) or 1 mM hydroxyectoine (OH-E), and their compatible solute contents were determined by HPLC as described in Materials and Methods. The data are averages of three different replicates, which on no occasion varied more than 5%.
