FIG. 1.

Organization of AIDA-I and of the MalE-AIDAc fusion. The organization of AIDA-I is represented by showing its signal sequence (SS) in white, the adhesin central domain in light gray, and AIDAc in dark gray. AIDAc has three subdomains: autochaperone, α helix (hatched box), and β barrel. The organization of the chimeric protein MalEβ is represented by showing MalE signal sequence (SS) in white, the mature portion of MalE in light gray, and AIDAc in dark gray. The boxes are not shown at scale. The amino acids of the chimeric protein are indicated at the junction of the different regions. The control protein, MalE*, is also represented, with the C-terminal extension generated by the procedure used to make the construct.