TABLE 2.

Description of the features of ExoU island B

EXB ORF	Annotation result(s)	Orientation	Start codon position	Stop codon position	Amino acid length	G+C (%)	Accession no. of closest homolog	Conserved domain search result
1	Hypothetical protein similar to PA0977	−	476	198	92	57	AAG04366	None
	TAGI repeat	N/Aa	200	219	N/A	45	N/A	N/A
2	Hypothetical protein similar to the C terminus of an integrase	−	952	443	169	61	AAP84129	None
3	Transposase-derived hypothetical protein	−	1142	957	61	54	ZP_00971320	None
4	Putative IS30 family transposase, N terminus	+	1498	2232	244	57	ZP_00463497	None
5	Putative IS30 family transposase, C terminus	+	2266	2847	193	55	ZP_00463497	Pfam00665
6	Transposase-derived hypothetical protein	−	3187	2813	124	61	ZP_00971320	None
7	Transposase-derived hypothetical protein	−	3435	3187	82	66	AAV82054	None
8	Putative transposase	−	3731	3432	99	60	ZP_00971321	Pfam01527
9	Hypothetical protein similar to PA0980	+	3918	4202	94	47	AAP82951	None
10	Hypothetical protein similar to PA0981	+	4239	4862	207	46	AAG04370	None
11	Hypothetical protein similar to RS08 from PAPI-2	−	5288	4968	106	50	AAP82953	None
12	Hypothetical protein similar to RS09 from PAPI-2	+	5400	5603	67	57	AAP82954	None
13	Hypothetical protein similar to RL019 from PAPI-1	+	5647	5877	76	55	AAP84146	None
14	Hypothetical protein similar to the C terminus of PA0982	−	6047	5874	57	60	AAP22574	None
15	GNAT family acetyltransferase	+	6091	6540	149	53	AAP82956	Pfam00583
16	ISPpu14 transposase Orf3, C terminus	−	7616	6543	357	59	NP_746094	Pfam03050
17	ISPpu14 transposase Orf3, N terminus	−	8122	7577	181	59	NP_746094	None
18	ISPpu14 transposase Orf2	−	8482	8142	111	64	NP_746095	Pfam05717
19	ISPpu14 transposase Orf1	−	8724	8479	81	58	NP_746109	None
20	Hypothetical protein similar to the N terminus of PA0982	−	9305	8859	178	61	AAG04371	None
21	Putative transposase similar to PA0983	+	9340	9627	95	57	AAG04372	Pfam01527
22	Colicin immunity protein similar to PA0984	+	10168	10494	108	41	AAP84212	None
23	Putative pyocin S5 similar to PA0985	−	12015	10519	498	46	AAP84213	None
24	Hypothetical protein	+	12348	12752	134	58	None	None
	IS407 left-terminal inverted repeat (69% identical)	N/A	13022	13070	N/A	53	N/A	N/A
25	Hypothetical protein similar to IS407 transposase OrfB and PA0987	+	13134	13856	240	62	ZP_00138569	Pfam00665
26	ExoU, type III effector protein	+	13981	16044	687	59	AAC16023	Pfam01734
27	SpcU, ExoU chaperone	+	16041	16454	137	56	AAC16024	None
28	RNA polymerase sigma-70 factor, extracytoplasmic function family	−	17275	16691	194	59	EAP51975	COG1595
29	Hypothetical protein	−	17705	17583	40	47	None	None
30	Hypothetical protein	−	18363	18157	68	54	None	None
31	Hypothetical protein	+	18670	19020	116	66	None	None
32	Putative oxygen-independent coproporphyrinogen III oxidase	+	19438	20862	474	63	EAP35559	COG0635
33	Putative nitric oxide reductase transcriptional regulator	−	22498	20864	544	68	NP_522520.1	COG3604
34	Putative quinol-dependent nitric oxide reductase	+	22625	24901	758	65	ABC27611	COG3256
35	Transposase-derived hypothetical protein	+	25262	25504	80	56	AAP82949	None
36	Conserved hypothetical protein	−	26346	25681	221	61	BAE50869	None
37	Putative regulator of mercury resistance proteins	+	26375	26902	148	57	AAD40337	cd01108
38	Hypothetical protein	−	27321	27163	164	60	None	None
39	Putative transcriptional regulator	−	27983	27558	141	49	EAP09715	COG1733
40	Hypothetical protein	+	27996	28118	40	52	None	None
41	NADH:flavin oxidoreductase family protein	+	28326	29462	378	54	ZP_00136272	cd02933
	TAGI repeat	N/A	29794	29813	N/A	45	N/A	N/A
42	PA0988	+	30024	N/A	N/A	N/A	N/A	N/A

^aN/A, not applicable.