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Effects of mutation in trh on expression of hrpG. A plasmid harboring hrpG::gus fusion genes inserted into the broad-host-range vector pHM1 (13) was introduced into T7174R and 74Trh::Kan. Each transformant was incubated in XOM2 and NBY for 15 h, and GUS activity per 108 CFU was measured. As a control, a plasmid harboring a gus-fused phosphoglucose isomerase gene (pgi), expression of which is independent of hrp (27), was used. Values are averages ± standard deviations (n = 3).
