TABLE 2.
Accumulation of trh and hpa1 transcripts under hrp-inducing (XOM2) and non-hrp-inducing (NBY) conditionsa
| Medium | Relative expression valueb
|
||
|---|---|---|---|
| gyrB | trh | hpa1 | |
| XOM2 | 104.1 ± 23.1 | 4.7 ± 0.6 | 24,863.7 ± 5,509.1 |
| NBY | 113.7 ± 67.1 | 5.4 ± 0.6 | 73.3 ± 28.2 |
a
Total RNA (10 ng) extracted from each bacterial strain after incubation in XOM2 was used as a template. The primers used for trh were 5′-AACAACTTGAAGCCGAAGG-3′ (sense primer) and 5′-CCACGTACTGCATGAAACC-3′ (antisense primer). Primers for detection of hpa1 and gyrB transcripts are given in Table 1, footnote a.
b
The threshold cycle (CT) was determined, and the relative expression value was calculated as 1/2CT × 108. Values are averages ± standard deviations for three independent experiments.