TABLE 1.
Strains, plasmids, and phages used in this study
| Bacterial strain, plasmid, or phage | Relevant feature | Source or reference |
|---|---|---|
| Strains | ||
| L. lactis subsp. cremoris | ||
| UC509.9 | Prophage-cured host for Tuc2009 | 9 |
| 901-1 | Lysogenic for phage TP901-1 | 4 |
| CSV61-1 | 901-1 lysogenic for TP901-1 bppU mutant | 32 |
| E. coli M15 | Host for pQE60 plasmids; contains pREP4, Kanr | QIAGEN |
| Bacteriophages | ||
| Tuc2009 | Temperate phage of L. lactis subsp. cremoris UC509 | 9 |
| TP901-1 | Temperate phage of L. lactis subsp. cremoris 901-1 | 4 |
| 48− | TP901-1 derivative with amber mutation in bppU gene | 32 |
| Plasmids | ||
| PQE60 | E. coli expression vector, Ampr | QIAGEN |
| PQ452009 | PQE60 + Tuc2009 orf45 | This study |
| PQ462009 | PQE60 + Tuc2009 orf46 | This study |
| PQ472009 | PQE60 + Tuc2009 orf47 | This study |
| PQ48C2009 | PQE60 + 1,914 bp (3′ end) of Tuc2009 orf48 | This study |
| PQ492009 | PQE60 + Tuc2009 orf49 | This study |
| PQ50N2009 | PQE60 + 540 bp (5′ end) of Tuc2009 orf50 (tal2009) | This study |
| PQ512009 | PQE60 + Tuc2009 orf51 | This study |
| PQ522009 | PQE60 + Tuc2009 orf52 | This study |
| PQ532009 | PQE60 + Tuc2009 orf53 | This study |
| PQ542009 | PQE60 + Tuc2009 orf54 | This study |
| PQ552009 | PQE60 + Tuc2009 orf55 | This study |