FIG. 2.

pspF transcription is inhibited by 6S RNA. The β-galactosidase activity of pspF-lacZ was measured in Miller units. (A) Wild-type (KW439) (gray line) or ssrS1 (KW440) (black line) cells containing pspF-lacZ were grown in unbuffered LB at 30°C to the cell densities indicated (OD₆₀₀). Data shown are from one representative experiment including three duplicates per time point. Similar results were obtained from three independent experiments. (B) Wild-type (KW439) or ssrS1 (KW440) cells containing pspF-lacZ and either no plasmid (white bars), pKK-6S* (solid bars), or pKK-6S(M5)* (shaded bars) were grown in unbuffered LB at 30°C for 18 h. Data shown are from one representative experiment with three duplicates. Similar results were seen in three independent experiments. Error bars, standard deviations from the averages.