FIG. 5.

6S RNA effects on cell survival at high pH require pspF. Wild-type (KW72), ssrS1 (GS075), ΔpspF (KW448), or ΔpspFssrS1 (KW449) cells were inoculated into LB pH 9.3 medium and grown at 37°C. At the times indicated, cell densities were determined by plating. Data shown are from one representative experiment including three duplicates per time point. Standard deviations from averages were all <6% and are too small to be visible here. Similar results were obtained from five independent experiments.