TABLE 3.
Genes differentially expressed in JAM1a
| Unique ID (GenBank) | Description | Microarray difference (n-fold) of geom means JAM1/UA159 | Microarray parametric P value | Real-time PCR fold difference of geom means JAM1/UA159 | Real-time PCR parametric P value | Real-time PCR primers |
|---|---|---|---|---|---|---|
| SMU.1596 | Putative PTS system, cellobiose-specific IIC component | 132.429 | P < 1E−07 | 1,111.11 | 0.002 | F-GGCTGGGCAATGAGTAATGG, R-TGACAAAACCAGGGATTAAAGC |
| SMU.1601 | Putative phospho-beta-glucosidase BglA | 100.611 | 5.83E−05 | |||
| SMU.148 | Putative alcohol-acetaldehyde dehydrogenase | 35.146 | 3.00E−07 | 15.4 | 0.031 | F-AACGGTTGGCATCATTGGTG, R-TGTTGGGTTAGTTGTTGGTACG |
| SMU.1598 | Putative PTS system, cellobiose-specific IIA component | 26.114 | 0.0021912 | 474.72 | 0.0087 | F-GAATTACAAGTTGCCGCATTTG, R-TCTGTGCTTGATGAGCTTTGAG |
| SMU.1411 | Conserved hypothetical protein | 23.54 | 3.40E−06 | |||
| SMU.936 | Putative amino acid ABC transporter, ATP-binding protein | 18.147 | 9.41E−05 | |||
| SMU.962 | Putative dehydrogenase | 13.452 | 0.000102 | |||
| SMU.934 | Putative amino acid ABC transporter, permease protein | 12.95 | 0.0005141 | 3.6 | 0.000062 | F-AGAAGCTGGTCTCAGTATTGG, R-ACCGATTGTATAGGCTAGAGC |
| SMU.1536 | Putative starch (bacterial glycogen) synthase | 12.283 | 4.70E−06 | |||
| SMU.935 | Putative amino acid ABC transporter, permease protein | 10.91 | 0.000406 | |||
| SMU.114 | Putative PTS system, fructose-specific IIBC component | 10.529 | 0.0022656 | 7.0 | 0.0067 | F-AGCCGACAAGGATGTTCAAGC, R-CTTTGCCAGCTAAAGCATCTGC |
| SMU.1537 | Putative glycogen biosynthesis protein GlgD | 10.407 | 1.95E−05 | 15.3 | 0.0046 | F-GCTATCGGATTCCCAGAAATGG, R-CACGACCGCTTCTGATATGATC |
| SMU.933 | Putative amino acid ABC transporter, periplasmic amino | 10.22 | 0.0008668 | |||
| SMU.496 | Putative cysteine synthetase A O-acetylserine lyase | 9.668 | 3.22E−05 | |||
| SMU.961 | Conserved hypothetical protein | 9.191 | 0.0008401 | 4.6 | 0.0048 | F-AACGGCTCTTGAGACTTATCG, R-TCTGGTGCCATTTGAATTTGC |
| SMU.180 | Putative oxidoreductase possible fumarate reductase | 8.948 | 0.0001778 | |||
| SMU.402 | Pyruvate formate-lyase | 8.655 | 1.03E−05 | 11.5 | 0.019 | F-AAGGAACTGACTGGAAAGAC, R-CGTGTTTCTTCGTAATGCG |
| SMU.2127 | Putative succinate semialdehyde dehydrogenase | 7.895 | 0.0017855 | |||
| SMU.149 | Putative transposase | 7.866 | 0.0017717 | |||
| SMU.1538 | Putative glucose-1-phosphate adenylyltransferase ADP-glucose pyrophosphorylase | 7.444 | 6.80E−06 | |||
| SMU.1535 | Glycogen phosphorylase | 7.129 | 9.36E−05 | |||
| SMU.312 | PTS system, sorbitol phosphotransferase enzyme IIBC | 6.326 | 4.50E−06 | 10 | 0.07 | F-TCGCTCTGGCTATTGTTGACTG, R-TGGGCTAAAGGTCCGCTCTTAC |
| SMU.1077 | Putative phosphoglucomutase | 5.515 | 1.37E−05 | 4.1 | 0.03 | F-ATTGGCGCTGGAACTAATCG, R-TTGAGAGAAATGACGGGAATCG |
| SMU.527 | Conserved hypothetical protein | 4.988 | 2.79E−05 | |||
| SMU.616 | Hypothetical protein | 4.842 | 6.19E−05 | |||
| SMU.1422 | Putative pyruvate dehydrogenase E1 component beta subunit | 4.835 | 0.0028314 | |||
| SMU.179 | Conserved hypothetical protein | 4.762 | 0.0005658 | 4.55 | 0.012 | F-ATGATTGTGGGCTGTTCG, R-CGTGTTCAGAGTGACCTAG |
| SMU.1603 | Putative lactoylglutathione lyase | 4.533 | 0.0022774 | |||
| SMU.508 | Conserved hypothetical protein | 4.498 | 0.0028192 | |||
| SMU.1013c | Putative Mg2+/citrate transporter | 4.46 | 0.0009883 | |||
| SMU.308 | Sorbitol-6-phosphate 2-dehydrogenase | 4.196 | 0.0010415 | |||
| SMU.1187 | Glucosamine-fructose-6-phosphate aminotransferase | 4.037 | 5.20E−05 | |||
| SMU.1254 | Conserved hypothetical protein | 4 | 0.0022115 | |||
| SMU.360 | Extracellular glyceraldehyde-3-phosphate dehydrogenase | 3.973 | 0.0011203 | |||
| SMU.675 | Phosphoenolpyruvate:sugar phosphotransferase system enzyme I, PTS system | 3.813 | 8.10E−06 | 1.6 | 0.014 | F-GCAGTAGATACCCTTGGTGAAG, R-TCTGTCACTTCTTTGAGAGCAC |
| SMU.460 | Putative amino acid ABC transporter, permease | 3.723 | 0.0001489 | |||
| SMU.426 | Copper-transporting ATPase P-type ATPase | 3.271 | 0.0007648 | |||
| SMU.882 | Multiple sugar-binding ABC transporter, ATP-binding protein, MsmK | 3.161 | 0.0006518 | |||
| SMU.361 | Phosphoglycerate kinase | 3.141 | 0.0001563 | |||
| SMU.674 | Phosphoenolpyruvate:sugar phosphotransferase system HPr | 3.066 | 0.0007686 | 1.7 | 0.01 | F-CATGCACGCCCAGCTACTTTG, R-CATCAGCACCTTGACCAACACC |
| SMU.1247 | Putative enolase | 2.851 | 0.0001529 | 0.48 | 0.07 | F-TGGTTCCTTCAGGAGCTTCTAC, R-CCGTCAAGTGCGATCATTGC |
| SMU.509 | Conserved hypothetical protein | 2.845 | 0.0006042 | 0.73 | 0.2 | F-GTTGGTCAGGTTCGACAAGC, R-TGGATACTTGGCAAAAGGATGG |
| SMU.1943 | Putative leucyl-tRNA synthetase | 2.643 | 0.0019886 | |||
| SMU.859 | Putative carbamoyl phosphate synthetase, small subunit | 2.373 | 0.0024238 | |||
| SMU.1115 | Lactate dehydrogenase | 2.332 | 0.000617 | |||
| SMU.1563 | Putative cation-transporting P-type ATPase PacL | 2.135 | 0.0014719 | |||
| SMU.1856c | Conserved hypothetical protein | 0.415 | 0.0015245 | |||
| SMU.613 | Hypothetical protein | 0.361 | 0.0018835 | |||
| SMU.1009 | Putative histidine kinase | 0.301 | 0.0007651 | |||
| SMU.2097 | Hypothetical protein | 0.295 | 0.0023821 | |||
| SMU.1516 | Putative histidine kinase CovS VicK homolog | 0.284 | 0.0004549 | 0.55 | 0.017 | F-CCTTAAACCGCCGTGAAAGTGG, R-AGGGCACCATCGTCCAAAGC |
| SMU.1432c | Putative endoglucanase precursor | 0.279 | 0.000974 | |||
| SMU.131 | Putative lipoate-protein ligase | 0.259 | 0.00074 | |||
| SMU.128 | Putative acetoin dehydrogenase (TPP-dependent), E1 component beta | 0.256 | 0.0015098 | |||
| SMU.1744 | Putative 3-oxoacyl-(acyl-carrier-protein) synthase III | 0.253 | 0.0013806 | 0.52 | 0.014 | F-AGTTATCGGTGCAGAAGTTC, R-AAAGGGTGACGAAACAGC |
| SMU.2042 | Dextranase precursor | 0.208 | 6.26E−05 | 0.23 | 0.003 | F-TTATTCCTGCAAACTCCTTAGC, R-ACCTCCAATAGCAGCATAACG |
| SMU.872 | Putative PTS system, fructose-specific enzyme IIABC component | 0.181 | 0.0003632 | 0.11 | 0.045 | F-TTAAGGCTGGGATCATGAATCG, R-AACAGGTTCACCATCAAGAGC |
| SMU.1926 | Putative transcriptional regulator | 0.177 | 0.0026323 | |||
| SMU.2038 | Putative PTS system, trehalose-specific IIABC component | 0.161 | 0.0003789 | 0.12 | 0.005 | F-GTATTGAAGGGGTCTCTAAGG, R-ATTACGAAATCCAAGAATCAGC |
| SMU.602 | Putative sodium-dependent transporter | 0.157 | 0.002202 | |||
| SMU.870 | Putative transcriptional regulator of sugar metabolism | 0.131 | 0.0007458 | |||
| SMU.871 | Putative fructose-1-phosphate kinase | 0.108 | 0.0004296 |
a
ID, identification number; geom, geometric; F, forward; R, reverse.