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Rates of oxygen consumption by the indicated enzyme combination
| Enzyme combination | Rate (mkat/kg of protein) of oxygen consumption induced bya:
|
|||
|---|---|---|---|---|
| Van | iVan | Ver | Pca | |
| VanA + IvaBb | 2.5 | 0.28 | 0.44 | 0.12 |
| IvaA + IvaB | 0.24 | 2.5 | 5.8 | 0.10 |
Assays were performed with NADH. Similar results were obtained with NADPH (data not shown).
The molar ratio of oxygenase to IvaB in these assays ranged from 5:1 to 10:1.
