FIG. 3.
DNA sequence in the promoter region of the gerK operon in wild-type strains PY79 and PS832. The translation initiation codon of gerKA, the first gene in the gerK operon, is in boldface italic letters, the putative gerKA ribosome-binding site is heavily underlined, and the putative −10 and −35 sequences of the gerK promoter are overlined. The two 5′ ends of gerKA mRNA determined as described in the text are indicated by the boldface nucleotides, with the more upstream G residue denoted as the primary transcript (+1 position). The consensus sequences for σF- and σG-dependent promoters are from reference 30. The abbreviations used in the consensus −10 and −35 sequences are the following: H, C or T; M, A, C or T; R, A or G; W, A or T; and X, any nucleotide.