TABLE 2.
Cells analyzed | Genotype | Relative peak mRNA levelsa
|
||
---|---|---|---|---|
gerAA | gerBA | gerKA | ||
PY79 strains | ||||
Sporulating PY79, peak 1 | Wild type | 0.3 | 1.0 (2 × 10−4) | 0.6 |
Sporulating PY79, peak 2 | Wild type | 0.3 | 0.9 | 1.0 (2 × 10−4) |
Sporulating SC500 | sigG | —b | —b | NDc (≤5 × 10−6) |
Sporulating IB1, peak 1 | spoVT | 0.5 | 0.5 | 1.0 (5 × 10−4) |
Sporulating IB1, peak 2 | spoVT | 1.0 (3 × 10−4) | 0.7 | 0.5 |
PS832 strains | ||||
Sporulating PS832, peak 1 | Wild type | 1.0 (4 × 10−4) | 0.5 | 0.6 |
Sporulating PS832, peak 2 | Wild type | 1.0 (4 × 10−4) | 0.4 | 0.5 |
IPTG induced PS766 | Pspac-sigG | 0.4 | 1.0 (1.3 × 10−3) | 0.9 |
ger mRNA levels are given relative to the mRNA whose level was the highest based on ratios of the level of mRNA to 16S rRNAs, and this level was set at 1.0. Values in parentheses are the actual ratios of the level of this ger operon's mRNA to the level of 16S rRNAs.
—, not analyzed.
ND, gerKA mRNA could not be detected in this strain.