TABLE 2.

Levels of gerAA, gerBA, and gerKA mRNAs in various types of B. subtilis cells^d

Cells analyzed	Genotype	Relative peak mRNA levelsa
		gerAA	gerBA	gerKA
PY79 strains
Sporulating PY79, peak 1	Wild type	0.3	1.0 (2 × 10−4)	0.6
Sporulating PY79, peak 2	Wild type	0.3	0.9	1.0 (2 × 10−4)
Sporulating SC500	sigG	—b	—b	NDc (≤5 × 10−6)
Sporulating IB1, peak 1	spoVT	0.5	0.5	1.0 (5 × 10−4)
Sporulating IB1, peak 2	spoVT	1.0 (3 × 10−4)	0.7	0.5
PS832 strains
Sporulating PS832, peak 1	Wild type	1.0 (4 × 10−4)	0.5	0.6
Sporulating PS832, peak 2	Wild type	1.0 (4 × 10−4)	0.4	0.5
IPTG induced PS766	Pspac-sigG	0.4	1.0 (1.3 × 10−3)	0.9

^ager mRNA levels are given relative to the mRNA whose level was the highest based on ratios of the level of mRNA to 16S rRNAs, and this level was set at 1.0. Values in parentheses are the actual ratios of the level of this ger operon's mRNA to the level of 16S rRNAs.

^b—, not analyzed.

^cND, gerKA mRNA could not be detected in this strain.

^dCells of various strains were grown and sporulated or induced with IPTG, and RNA was isolated and quantitated as described in the text. Values shown are from RNA samples that gave peak levels of ger operon mRNAs as seen in Fig. 1 and 2.