TABLE 3.
Viability of spores of various strains prepared with or without DPAa
| Strain (genotype) | Sporulated with DPA | CFU/ml of spores at an OD600 of 1 as determined by various methods for the assessment of spore viabilityb
|
||
|---|---|---|---|---|
| Nutrients | CaDPA | Decoat/lysozyme | ||
| FB122 (sleB spoVF) | Yes | 108 | 108 | 107 |
| FB122 (sleB spoVF) | No | 104 | 107 | 9 × 106 |
| PS3664 (sspA sspB sleB spoVF) | Yes | 9 × 107 | 108 | 8 × 106 |
| PS3664 (sspA sspB sleB spoVF) | No | <103 | <10−3 (<10−3) | <10−3 (<10−3) |
| PS3747 (cotE sspA sspB sleB spoVF) | No | NT | <10−3 | <10−3 |
a
Spores of strains FB122 and PS3664 were prepared on plates at 37oC with or without DPA and were cleaned as described in Materials and Methods. The viability of the cleaned spores was measured by plating on LB plates alone (Nutrients), on LB plates after pretreatment with Ca2+-DPA (CaDPA), or on LB plates after decoating and germinating with lysozyme in hypertonic medium (Decoat/lysozyme).
b
NT, not tested. Values in parentheses are for spores prepared in liquid medium at 37°C and then cleaned as described in Materials and Methods.