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Growth deficiency in liquid culture of iron-starved ΔfslA mutant. Washed cells of LVS and GR7 from a 24-h culture in CDM were grown for 24 h in iron-limiting che-CDM to deplete internal iron stores. These cultures were then inoculated into fresh iron-replete or iron-limiting che-CDM, and the growth was plotted. Cultures were grown in triplicate, and the means and standard deviations are depicted.
