Cell morphology and location and distribution of RNA polymerase in cells carrying a plasmid-borne fragment of the rrnB operon transcribed from different promoters. (A, B, and C) Plasmid pDJ2754-11 (no promoter). (D, E, and F) Plasmid pDJ2754-17 (rrnB P1 promoter). (G, H, and I) Plasmid pDJ2754-15 (rrnB P2 promoter). (J, K, and L) Plasmid pDJ2845 (tac promoter). (A, D, G, and J) Merged phase-contrast and DAPI fluorescence images. (B, E, H, and K) Fluorescence from the RNAP-GFP fusion protein. (C, F, I, and L) Overlays of the fluorescence from the RNAP-GFP fusion protein (green) and the fluorescence of the DAPI-stained nucleoids (red).