FIG. 3.
Cbm2-containing proteins specifically bind to Avicel. (A) SDS-11% PAGE gel. Sample proteins were incubated with BSA-treated Avicel as described in Materials and Methods. Arrows indicate test protein bands. Lanes: 1, Cbm2A:His6; 2, Cbm2B:His6; 3, pepsin. Porcine lipase and ovalbumin controls are not shown. (B) SDS-8% PAGE gel prepared as in panel A. Lanes: 1, rCbm2C; 2, pepsin; 3, porcine lipase. The ovalbumin control is not shown. For reasons yet to be determined a number of cloned 2-40 proteins, including Cbm2B and Cbm2C, consistently run higher in SDS-PAGE than their predicted molecular masses.