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Strains with three (TGW1) or five (TGW2) GATC sites eliminated from the dnaA promoter initiate DNA replication synchronously. Cells growing exponentially in M9 enriched (rich) or M9 minimal (MM9) medium were treated with rifampin and cephalexin for 4 h. Samples were removed, fixed, stained, and analyzed by flow cytometry as described previously (19).
