TABLE 2.
Strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| DH5α | supE44 lacU169 [φ80 lacZΔM15] hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Stratagene |
| JZ294 | F−polA1 argH hsdR rpsL thyA36 | 13 |
| JG108 | metE70 thyA deoC2 lacZ lacY14(Am) rhaS rpsL λ− | Cathy Joyce |
| JZ365 | JG108 polA12::Tn10 | Cathy Joyce |
| JZ366 | JG108 polA107::Tn10 | Cathy Joyce |
| JZ1101 | W3110 tnaA262::Tn10 | Charles Yanofsky |
| MG1655 | λ− F− | Mary Berlyn |
| TGW1 | MG1655 with three GATC sites downstream of dnaAp2 eliminated | This study |
| TGW2 | MG1655 with five of six GATC sites in dnaA promoter region eliminated | This study |