FIG. 4.
Germination of spore suspensions in AGFK and l-alanine solutions. Spores prepared from cells of 168 (squares), MM1325 (ΔprpE) (triangles), and MM1502 (ΔprpE amyE::pspac-prpE) (circles) grown in NB medium were heat activated and subsequently incubated in 10 mM Tris-HCl (pH 7.5) with 3.3 mM l-asparaginate, 5.6 mM d-glucose, 5.6 mM d-fructose, and 10 mM KCl (A and C) or with 10 mM l-alanine (B and D). IPTG added to the germination solution alone has no effect on germination. (A and B) Germination was followed by measuring the A600 of the spore suspension. (C and D) Release of dipicolinic acid was followed by measuring the A280 of the spore suspension.