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Expression of the sspB gene, coding for the small acid-soluble protein, in the wild-type strain (wt) and a strain in which prpE was deleted. Bacteria were grown in Sterlini-Mandelstam medium at 37°C with shaking. At time zero, sporulation was initiated by replacing Sterlini-Mandelstam growth medium with sporulation medium. Total RNA was isolated after 3 h after the change of medium. The quantitative RT-PCRs were standardized relative to sigA mRNA.
