TABLE 1.
B. subtilis strains used in this study
| Strain | Genotype | Reference, source, or constructiona |
|---|---|---|
| 168 | trp | 1 |
| MM1325 | ΔprpE Spcr | 11 |
| BFA2853 | prpE::pMutin4 Eryr | Our collection |
| MM1453 | amyE::pSpac-prpE Catr | pKH18→168 |
| MM1502 | ΔprpE amyE::pSpac-prpE Catr Spcr | MM1325→MM1453 |
| MM1503 | prpE-gfp Catr | pKH21→168 |
| MO1615 | sigH::Kanr | From P. Stragier |
| 719 | trp2 pheA1 sigF::Kanr | From P. Stragier |
| RL1061 | sigE::Ermr | From R. Losick |
| 1446 | trp2 pheA1 sigG::Tetr | From P. Stragier |
| MM1504 | sigH::Kanr | MO1615→168 |
| MM1505 | trp2 pheA1 sigF::Kanr | 719→168 |
| MM1506 | trp2 pheA1 sigG::Tetr | 1446→168 |
| MM1601 | sigE::Ermr | RL1061→168 |
| MM1602 | sigH::KanrprpE-gfp Catr | MM1504→MM1503 |
| MM1507 | sigF::Kanr prpE-gfp Catr | MM1505→MM1503 |
| MM1508 | sigE::ErmrprpE-gfp Catr | MM1601→MM1503 |
| MM1509 | sigG::Tetr prpE-gfp Catr | MM1506→MM1503 |
| PS1245 | gerA-lacZ Catr | From P. Setlow |
| PS3441 | psspB-gerA MLSr | From P. Setlow |
| FB58 | psspB-gerB Spcr | From P. Setlow |
| PS3461 | psspB-gerK MLSr | From P. Setlow |
| MM1603 | gerA-lacZ Catr | PS1245→168 |
| MM1604 | psspB-gerA MLSr | PS3441→168 |
| MM1605 | gerA-lacZ ΔprpE Spcr Catr | MM1602→MM1325 |
| MM1606 | psspB-gerA amyE::pSpac-prpE MLSr Catr | MM1603→MM1453 |
| MM1607 | psspB-gerA ΔprpE MLSr Spcr | MM1603→MM1325 |
| MM1608 | psspB-gerB Spr | FB58→168 |
| MM1609 | psspB-gerK MLSr | PS3461→168 |
| MM1610 | psspB-gerB amyE::pSpac-prpE Spcr Catr | MM1608→MM1453 |
| MM1611 | psspB-gerB prpE::pMutin4 Eryr Spcr | BFA2853→MM1320 |
| MM1612 | psspB-gerK amyE::pSpac-prpE MLSr Catr | MM1609→MM1453 |
| MM1613 | psspB-gerK ΔprpE MLSr Spcr | MM1609→MM1325 |
| MM1615 | amyE::gerB-bgaB Catr | pKH26→168 |
| MM1616 | amyE::gerB-bgaB ΔprpE Catr Spcr | MM1615→MM1325 |
| MM1618 | amyE::gerK-bgaB Catr | pKH25→168 |
| MM1619 | amyE::gerK-bgaB ΔprpE Eryr Spcr | MM1618→MM1325 |
| MM1621 | sspB-lacZ Eryr | pKH24→168 |
| MM1622 | sspB-lacZ ΔprpE Eryr Spcr | MM1621→MM1325 |
| MM1624b | PprpE-gfp Eryr | pKH27→168 |
a
Strains were constructed by transformation of plasmid or chromosomal DNA from the strain to the left of the arrow into the strain to the right of the arrow.
b
Strain containing transcription fusion for gfp from the prpE promoter.