B. burgdorferi cytoplasmic proteins purified by affinity chromatography using erpG 5′ noncoding DNA as bait. Proteins in elutions 1, 2, and 3 were eluted with NaCl at concentrations of 500, 750, and 1,000 mM, respectively. Proteins were separated by SDS-PAGE and visualized with SYPRO-Ruby. Numbers on the right indicate positions of molecular mass standards.