Comparison of the structures of the lowest-energy structure of ɛ186-bound θ (A), the lowest-energy uncomplexed θ structure (B), and the lowest-energy HOT structure (C). All structures have the same overall fold, but the angles between helices 1 and 3 are different. The ribbons trace the backbones of ɛ186-bound θ, free θ (PDB code 2AE9), and HOT (PDB code 1SE7), respectively.