TABLE 3.
Effect of overexpression of full-length and truncated GlnD proteins on expression of glnKp-lacZ in strain AT5 (ΔglnD99::Tn10 recA56)
| Plasmid | Description of GlnD | β-Galactosidase activitya (Miller units) when grown in nitrogen response mediumb with:
|
|
|---|---|---|---|
| NH4+ | Gln | ||
| None | None | 4 | |
| pAT33 | Full length | 460 ± 120 | 2,600 ± 170 |
| pAT25 | Truncated, GlnD42 | 1,700 ± 230 | 2,700 ± 220 |
| pAT24 | Truncated, GlnD39 | 1,600 ± 230 | 2,700 ± 150 |
a
Values are means of triplicate determinations from two to five separate cultures ± standard deviations.
b
Medium contained 0.2% (wt/vol) (NH4)2SO4 or glutamine.