FIG. 2.
Electronic absorption spectra of 3-Cl HOPDA (solid line) and its BphK-catalyzed transformation product (dotted line). The product was generated by incubating 0.1 mM 3-Cl HOPDA with 1 mM GSH and an excess of BphK under standard reaction conditions for 2 min. 3-Cl HOPDA and the reaction product were injected into the HPLC. Spectra were collected in a 50:50 mixture of methanol and water (0.5% H3PO4) using a Waters 2996 photodiode array detector during HPLC analysis. The retention time and spectrum of the reaction product are identical to those of HOPDA.