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. 2006 Jun;188(12):4424–4430. doi: 10.1128/JB.01849-05

FIG. 3.

FIG. 3.

Steady-state transformation of 3-Cl HOPDA by BphK. The main panel depicts the dependence of initial velocity on the concentration of 3-Cl HOPDA in the presence of 1 mM GSH. The curve represents a best fit of the Michaelis-Menten equation to the data using the least-squares and dynamic-weighting options of LEONORA. The fitted parameters are a value for Inline graphic of 22 ± 2 μM and a velocity of 41 ± 2 μM/min. The insert depicts the dependence of initial velocity on the concentration of GSH using 50 μM 3-Cl HOPDA. The fitted parameters are a value for Inline graphic of 120 ± 20 μM and a velocity of 32 ± 3 μM/min. Experiments were performed using potassium phosphate, pH 6.5 (I = 0.05 M), at 25°C.