TABLE 3.
Properties of mobile-element insertions
| Mobile element | Copy no. in published sequencea | No. of mutants | Direct repeats (bp)a | Aligned target sequencesb | Possible donating copies |
|---|---|---|---|---|---|
| SM2 | 25 | 1 | 6 | 148: ATAGAGAAAGCAACATTGGAGATTCTTAACGGTG | 1980139 |
| ISC1058 | 14 | 66 | 9 | 20 (9) × 535912: TAAATTCTTTTTCACAGAGTCTCTA | Ah/Bh/2811580 |
| 37 (9) × 535914: AATTCTTTTTCACAGAGTCTCTACG | A/B/2811580 | ||||
| 9 (6) × 535952: ATAATTTCCTTTC-GAGTAAGACTTC ms | A/B/2811580 | ||||
| ISC1078 | 8c | 2 | 2 | 009: TTTCAGACGCACTAG-TAATCATAGATAGACAAG | 35310/1088786i/2734629 |
| 110: AATTTTACGATATAGTTAATCA-AGCTATA-AAGA | 1711152/1903184i/1960125i | ||||
| ISC1217 | 11 | 4 | 6 or 7 | 105: GTCAATTCTAG-ATTATTTGG | 1892826 |
| 3 (1) × 536422: AGAATTTCT-GAAATTTTAAA ms | 838162 | ||||
| P1: ATTAGAACTCG-ATGTAAAAGd | −d | ||||
| SM2: AAGA-ATCTCCAATGTTGCTT ms | 1980139 | ||||
| ISC1225 | 11 | 3 | 4 or 5 | 101: CATAGTCTCTCCGGGTATGGGTAGTCAAGGTGGAe | 1362900 |
| 104: CATAGTCTCTCCGGGTATGGGTAGTCAAGGTGGAe | 2476985 | ||||
| 154: CATAGTCTCTCCGGGTATGGGTAGTCAAGGTGGAe | 1362900 | ||||
| ISC1359 | 10c | 11 | 4 | 4 (4) × 535928: CAGAGTCTCTACGTATGAATTTCGf | 445747/1300529i/1671315 |
| 7 (5) × 536659: AATTTCCTCCACGTCTAAACCAAT ms | 445747 | ||||
| 003 at 539558: AATAGCTTCAACGT-TAAAACTTGA ms | See the text and Fig. 3 | ||||
| P1 at 536969: TATAGGAGAGACTTCGAAAAAATGd | −d | ||||
| ISC1439 | 32c | 7 | 9 | 121: TACATAATGAAAAGCATAGTTGAAAGATTATCT | 454065-480919j |
| 129: AGAATGTTGAAAAGTAGAGT---AATATTAGCAATg | 2595886 | ||||
| 170: TGAAGAGTGATAAACTTAAGGATAATGAAAAGAAG | 2595886 | ||||
| 2 (2) × 536523: GAAAA--GTAGAGTAATATTAGCAATGGATAAACC | 670424/1418678/2903467 | ||||
| 147: TAAATTTTTAGAGT-TTGC-ATCAAGATATCTTTT ms | 454065-480919j | ||||
| 165: CTGCATTATAAATACTTCTTCCAATGATTTC-AT ms | 454065-480919j | ||||
| P1: ATTCATC-TA-GAATTTCTG-AAAT--TTTAAATAd ms | −d |
This study and reference 3.
The sequences duplicated to form direct repeats are underlined. With single insertions at a site, the mutant strain number is shown, and with multiple insertions, the number of insertions at each position is given, with the minimum number of independent insertions in parentheses. Bases that are identical in more than 66% of the target sites are in italics, except for ISC1439, where the italics are for pairwise identity. ms, the minus strand is used for the alignment.
An IS element copy was missing from one or more of the sites (see the text).
Insertions in pyrEF of S. solfataricus P1, where donor information is unavailable (from reference 16).
All three insertions at the same position, and the 4-bp and 5-bp DRs, are shown with double and single underlining, respectively.
The pyrE start codon is marked in boldface.
The pyrF start codon is marked in boldface.
Groups of identical ISC1058 copies: A, 1663758/1703044/1847493/2968643; B, 1042703/1050863.
This IS element copy is not present in the S. solfataricus P2A strain (see the text).
The donating copy is probably a recombination product of ISC1439 at positions 454065 and 480919 (see the text).