FIG. 2.

Distribution of the nucleoid sizes following the overproduction of MukBEF and MukB. DH5α cells harboring plasmid pBB10 or pBB03 were supplemented with arabinose (Ara) or glucose (Glu) as appropriate to repress or induce the overproduction of MukB (B) or MukBEF (BEF), further incubated for 3 hours at 37°C in LB, and observed by fluorescence microscopy. Nucleoid size was measured using Nucleus, the nucleoids were binned according to their sizes, and the number of nucleoids in each bin was normalized to the total number of scored nucleoids (between 430 and 700 nucleoids for each data set). The resulting distributions are shown on the plot. The arrow indicates the sizes of single nucleoids found in short, nonoverproducing cells.