FIG. 3.

Growth curves, extent of chromosome condensation, and rates of macromolecular synthesis in cells that overproduce MukBEF or MukB. DH5α cells harboring pBAD, pBB10 (pB), or pBB03 (pBEF) were induced with arabinose in the synthetic medium, and the rate of (left panels) or cumulative (right panels) incorporation of radioactivity into protein, RNA, or DNA was measured as described in Materials and Methods. Synthesis rates are expressed as the amounts of acid-insoluble radioactivity accumulated after a 10-min pulse, normalized to the total amount of radioactivity in the labeling mixture and further normalized to the OD₆₀₀ of the cell culture. Cumulative (total) incorporation is measured as the fraction of radioactivity found in acid-insoluble form. The plotted data represent the averages for two independent experiments; error bars show standard deviations. For convenience, the extent of chromosome condensation in M9CA medium is also shown (Fig. 5). The appropriate growth curves are shown in the top panels.