FIG. 4.
Comparison of 32P-labeled lipid A (A) and phospholipid fractions (B) isolated from H. pylori 26695 and 26695/lpxEHP::cm. 32P-labeled lipid A species or phospholipids were isolated as described in Materials and Methods and separated by TLC using the solvent chloroform, pyridine, 88% formic acid, and water (50:50:16:5 [vol/vol/vol/vol]). The 32P-labeled lipids were visualized by phosphorimaging. The identities of the major phospholipids are based upon the major phospholipids reported for H. pylori (24) and the migration of the major phospholipids found in E. coli (72). PG, phosphatidylglycerol; PE, phosphatidylethanolamine; CL, cardiolipin.