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Site specificity of insertion in E. faecalis JH2-2. (A) Lanes: M, 100-bp benchtop ladder; 1, PCR of the left junction; 2, PCR of the right junction; 3, PCR of the circular-form junction; 4, PCR of the empty target site. (B) Schematic showing the positions of the primers for each PCR. The unfilled box represents the transposon. The filled box represents the 18-bp repeat sequence.
