TABLE 2.
Primers used in this study
| Primer | Sequence 5′→3′ | Amplicona |
|---|---|---|
| GWEF1 | AACTTAAACTGATACCTGTGTGGTCAG | sspPCR of BAC H12; excisionase- and integrase-containing fragment |
| GWEF2 | GATACCTTGTTTCATAAGGGATTAGCC | sspPCR of BAC H12 |
| GWEF3 | TATGGAAGGAGGAAAAACGA | sspPCR of BAC H12 |
| GWEF4 | CCATTCCGTATTGTCAGAAAC | sspPCR of BAC H12 |
| GWEF5 | GCATATGACTGTTCATGG | sspPCR of BAC H12; right genome-Tn junction, CI joint |
| GWEF8 | CCCATTCTACACTGGTCGTC | Right genome-Tn junction; target site-, excisionase-, and integrase-containing fragment |
| GWEF7 | GTTCGATTAAAGACTACCAG | Left genome-Tn junction; CI joint |
| GWEF9 | ATACGGTATACGGACTAGCTATAC | Left genome-Tn junction; target site |
a
CI, circular intermediate; Tn, transposon.