FIG. 4.

Western blot analysis of plasmid-encoded P. aeruginosa 1244 pilin. Cell extracts were prepared from overnight plate cultures in which induction of pilAO₁₂₄₄ (pPAC46) or pilA₁₂₄₄ (pPAC24) was accomplished by the addition of 5 mM IPTG to the medium unless indicated otherwise. The blot was probed with 1244 pilin-specific MAb 6.45. G is glycosylated 1244 pilin, and NG is nonglycosylated 1244 pilin. The dotted line was drawn from the centers of the NG pilin and the PA103/pPAC24 pilin bands (also nonglycosylated) to better illustrate the difference in molecular weight between the pilins shown on this blot.