. 2006 Jun;188(12):4244–4252. doi: 10.1128/JB.00273-06

TABLE 1.

P. aeruginosa strains and plasmids used in this study

P. aeruginosa strain or plasmid	Description^a	Source or reference
Strains
PA103	Wild type; IATS O11	30
1244N3	rpoN (Tc^r); IATS O7	40
PA103 wzy::aacC1	wzy (Gm^r)	13
PA103 wbjA::aacC1	wbjA (Gm^r)	12
PA103 wbjE::aacC1	wbjE (Gm^r)	This study
Plasmids
pRK2013	Km^r; helper plasmid for triparental mating	42
pLPS2	Broad-host-range cosmid containing the P. aeruginosa PA103 O11 gene cluster	22
pEX100T	5.8-kb gene replacement vector, oriT, sacB⁺; Ap^r	47
pUCGm	Contains a Gm^r cassette (aacC1); Ap^r, Gm^r	46
pEX100T-wbjE::aacC1	pEX100T with insertion of the Gm^r cassette (aacC1) in wbjE	This study
pUCP18	pUC18-derived broad-host-range shuttle vector; Ap^r/Cb^r	45
pCD207	pUCP18 with P. aeruginosa PA103 wbjE	This study
pMMB66EH	8.8-kb broad host range expression vector; Ap^r/Cb^r	21
pPAC46	pMMB66EH with P. aeruginosa 1244 pilAO	6
pPAC24	pMMB66EH with P. aeruginosa 1244 pilA	6

Ap^r, ampicillin resistance; Cb^r, carbenicillin resistance; Tc^r, tetracycline resistance; Gm^r, gentamicin resistance; Km^r, kanamycin resistance.