TABLE 2.
Plasmids used in this worka
| Plasmid | Relevant features | Reference |
|---|---|---|
| pBT205 | Vector for integration into B. subtilis chromosome, promoterless lacZ gene, ColE1 ori Apr Cmr | 14 |
| pBT205O+E | pBT205 with ω gene with its promoter, Pω, and part of the ɛ gene | This work |
| pBT233 | pSM19035 derivative, accession no. X64695 | 11 |
| pBT346 | pHP13 with δ, ω, ɛ, and ζ genes (pDB101 coordinates 4696 to 8271), HincII-HincII pBT233 fragment cloned into SmaI siteb | 11 |
| pBT346-5 | pHP13 with ω gene (pDB101 coordinates 6312 to 6894) (sixth triplet mutated into a stop codon)b | 14 |
| pDO1000 | pUC18 with δ and ω genes (pDB101 coordinates 5382 to 6740)b | This work |
| pDO1030 | pUC18 with δ and ω genes, with δ truncated after the 23rd codon | This work |
| pDO1040 | pUC18 with δ and ω genes, with δ truncated after the 40th codon | This work |
| pDO1212 | pUC18 with δ gene promoter (Pδ) | This work |
| pDO1340 | pUC18 with δ and ω genes, with δ's Walker motif A deleted | This work |
| pDO3000 | pUC18 with δ and ω genes, with δ promoter (Pδ) replaced with copS promoter (PcopS) | This work |
| pDO3212 | pUC18 with copS gene promoter (PcopS) | This work |
| pDO7000 | pUC18 with δ and ω genes, with δ promoter (Pδ) replaced with ω promoter (Pω) | This work |
| pDO7212 | pUC18 with ω gene promoter (Pω) | This work |
| pHP13 | Shuttle vector for E. coli/B. subtilis, with high copy number in E. coli and low copy number in B. subtilis, ColE1 ori/pTA1060 ori Err Cmr | 25 |
| pHP1000 | pHP13 with δ and ω genes | This work |
| pHP1340 | pHP13 with δ and ω genes, with δ's Walker motif A deleted | This work |
| pHV1436 | Shuttle vector for E. coli/B. subtilis, with high copy number in E. coli and low copy number in B. subtilis, ColE1 ori/pTB19 ori Apr Cmr | 30 |
| pOSE10 | pBT205 with inactivated ω gene with its promoter, Pω, and part of the ɛ gene | This work |
| pOSZ5 | pHP13 with inactivated ω gene with its promoter, Pω, and the ɛ and ζ genes | This work |
| pRC1 | pDB101 derivative carrying copS with its promoter and the repS and erm genes | 14 |
| pRP346-5 | pHP13 with δ, ω, ɛ, and part of the ζ gene (pDB101 coordinates 4693 to 7251)b | Laboratory collection |
| pUC18 | E. coli general cloning vector, ColE1 ori Apr | 51 |
| pUCRC | pUC18 with pRC1 fragment encompassing repS, α, and β genes (pDB101 coordinates 20 to 4103)b | This work |
| pUCRS | pUCRC derivative with HindIII site in repS gene converted into a StuI site | This work |
| pUHC13 | Shuttle vector for E. coli/B. subtilis, with high copy number in E. coli and low copy number in B. subtilis, ColE1 ori/pTB19 ori Apr Cmr | This work |
| pUHC1000 | pUHC13 with δ and ω genes | This work |
| pUHC1340 | pUHC13 with δ and ω genes, with δ's Walker motif A deleted | This work |
| pUHC1212 | pUHC13 with δ gene promoter (Pδ) | This work |
| pUHC3212 | pUHC13 with copS gene promoter (PcopS) | This work |
| pUHC7212 | pUHC13 with ω gene promoter (Pω) | This work |
| pUSE2 | Shuttle vector for E. coli/B. subtilis, with high copy number in E. coli and low copy number in B. subtilis, ColE1 ori/pSM19035 ori Apr Emr | This work |
| pUSE1000 | pUSE2 with δ and ω genes | This work |
| pUSE1030 | pUSE2 with δ and ω genes, with δ truncated after the 23rd codon | This work |
| pUSE1040 | pUSE2 with δ and ω genes, with δ truncated after the 40th codon | This work |
| pUSE1340 | pUSE2 with δ and ω genes, with δ's Walker motif A deleted | This work |
| pUSE3000 | pUSE2 with δ and ω genes, with δ promoter (Pδ) replaced with copS promoter (PcopS) | This work |
| pUSE7000 | pUSE2 with δ and ω genes, with δ promoter (Pδ) replaced with ω promoter (Pω) | This work |
a
Plasmid construction details are given in the text.
b
pDB101 is a pSM19035 derivative which contains the two long inverted repeats and possesses all functions associated with replication, copy control, stable maintenance, and antibiotic resistance (accession no. X66468).