TABLE 4.

Regiospecificity of butane oxidation by P. butanovora mutant strains

P. butanovora strain	Rate of product accumulation (nmol min−1) (mg protein−1) ± SD		Ratio of 2-butanol to 1-butanol accumulation	Fold increase over Rev WT accumulation
	1-Butanol	2-Butanol
Rev WT	74.0 ± 3.9	2.70 ± 0.91	0.04
T148C	41.3 ± 4.0	6.14 ± 0.89	0.15	3.7
Q320K	50.4 ± 7.8	10.3 ± 0.53	0.20	5.6
F321Y	102.4 ± 5.7	NDa	NAb	NA
L279F	47.0 ± 7.1	10.45 ± 4.2	0.22	5.5
G113N	1.77 ± 0.06	19.67 ± 2.4	11.1	277.5

^aND, none detected.

^bNA, not applicable.