TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Genotype or descriptiona	Reference or source
P. pseudoalcaligenes strains
KF707	Bph+, wild type	9
KF707ΔR1	Bph−, bphR1::Tcr	33
KF707ΔR2	Bph−, bphR2::Tcr	32
KF707R2+	KF707ΔR2 carrying pTWF17 containing bphR2; Bph+ Apr Kmr	32
E. col strains
JM109	Host strain for DNA manipulation	Takara Shuzo
BL21(DE3)	Host strain for expression	Novagen
Plasmids
pTWF3	0.75-kb NcoI-SalI PCR fragment in pET32b(+); bphR1	33
pTWF17	1.8-kb EcoRI fragment containing bphR2 coding sequence in pMMB66EH; Apr	32
pTWF21	0.95-kb NcoI-KpnI fragment from KF707 in pTV118N; bphR2	32
pGEM-T Easy	Cloning vector; Apr	Promega
pFHF10	161-bp PCR fragment in pGEM-T Easy, including bphR1 promoter	This study
pFHF11	123-bp PCR fragment in pGEM-T Easy, including bphA1 promoter	This study
pFHF12	183-bp PCR fragment in pGEM-T Easy, including salA promoter	This study
pFHF13	585-bp PCR fragment in pGEM-T Easy, including intervening region of bphR2 and salA	This study
pHGS396-xylE	0.4-kb fragment containing xylE coding sequence from pWWO; Cmr	This study

^aBph⁺, able to grow on biphenyl as a sole carbon source; Bph⁻, unable to grow on biphenyl as a sole carbon source; Ap^r, resistance to ampicillin; Tc^r, resistance to tetracycline; Km^r, resistance to kanamycin; Cm^r, resistance to chloramphenicol.