TABLE 1.
Strains used in this studya
| Strain | Characteristics | P1 transduction or conjugation | Reference or source |
|---|---|---|---|
| CC101 | Derivative of strain P90C [araA(lac proB)xIII] carrying F′ lacIZ- proB+; lacZ has a mutation (GAG to TAG) at codon 461 | 7 | |
| CC104 | Derivative of strain P90C [araA(lac proB)xIII] carrying F′ lacIZ- proB+; lacZ has a mutation (GAG to GCG) at codon 461 | 7 | |
| AR30 | ΔdinB61::ble sulA211 | 4 | |
| DE2302 | thr-1 ara-14 leuB6 Δ(gpt proA)62 lacY1 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 arg3 thi-1 uvrA6 Δ(umuDC)595::cat fadR615::Tn10 purB58 | 34 | |
| EC8 | thr-1 ara-14 leuB6 Δ(gpt-proA)62 lacY1 tsx-33 supE44 galK2 hisG4 rpsL31 xyl-5 mtl-1 argE3 thi-1 uvrA6 Δ(umuDC)596::ermGT fadR+ purB+ | 11 | |
| KY1056sFtet101 | AB1157 derivative; harboring F′ derived from CC101, which has Tn10 in it for selection of F′ | K. Yamamoto | |
| KY1056sFtet104 | AB1157 derivative; harboring F′ derived from CC104, which has Tn10 in it for selection of F′ | K. Yamamoto | |
| YG6125A | AB1157 derivative; harboring F′ derived from CC101, which has Tn10 in it for selection of F′ and ΔdinB::kan | This study | |
| YG6125B | AB1157 derivative; harboring F′ derived from CC104, which has Tn10 in it for the selection of F′ and ΔdinB::kan | This study | |
| QC1736 | Δ(argF-lac)U169 rpsL ΔsodA3 sodB::MudPR fur::kan; Cmr Kmr | 29 | |
| YG6177 | Like QC1736 but ΔdinB61::ble; Cmr Kmr Zcr | AR30 (P1) → QC1736 | This study |
| YG6180 | Like QC1736 but ΔumuDC(596)::ermGT; Cmr Kmr | DE2302/EC8 (P1) → QC1736 | This study |
| YG6124 | Like QC1736 ΔdinB61::ble; ΔumuDC(596)::ermGT; Cmr Kmr Zcr | DE2302/EC8 (P1) → YG6177 | This study |
| YG6175b | Like QC1736 but harboring F′ from CC101; Cmr Kmr Tcr | KY1056sFtet101 → QC1736 | This study |
| YG6176b | Like QC1736 but harboring F′ from CC104; Cmr Kmr Tcr | KY1056sFtet104 → QC1736 | This study |
| YG6178b | Like QC1736 but ΔdinB61::ble and harboring F′ from CC101; Cmr Kmr Tcr Zcr | YG6125A → YG6177 | This study |
| YG6179b | Like QC1736 but ΔdinB61::ble and harboring F′ from CC104; Cmr Kmr Tcr Zcr | YG6125B → YG6177 | This study |
| YG6181b | Like QC1736 but ΔumuDC(596)::ermGT and harboring F′ derived from CC101; Cmr Kmr Tcr | KY1056sFtet101 → YG6180 | This study |
| YG6182b | Like QC1736 but ΔumuDC(596)::ermGT and harboring F′ from CC104; Cmr Kmr Tcr | KY1056sFtet104 → YG6180 | This study |
| YG6126b | Like QC1736 but ΔdinB61::ble and ΔumuDC(596)::ermGT and harboring F′ from CC101; Cmr Kmr Tcr Zcr | YG6125A → YG6124 | This study |
| YG6127b | Like QC1736 but ΔdinB61::ble and ΔumuDC(596)::ermGT and harboring F′ from CC104; Cmr Kmr Tcr Zcr | YG6125B → YG6124 | This study |
The deletion strains for dinB encoding DNA Pol IV were constructed by P1 transduction as indicated. The umuDC deletion encoding DNA Pol V was introduced into QC1736 and YG6177 by two-step P1 transduction (11). (P1) indicates that P1vir phage lysate was prepared in the strain. F′ with a mutation for specific detection of changes from G · C to T · A or from A · T to C · G was separately introduced by conjugation as indicated. The arrows indicate the directions of transfer for P1 transduction and conjugation. Chloramphenicol, kanamycin, tetracycline, and zeocin were used at concentrations of 10 μg/ml, 25 μg/ml, 10 μg/ml, and 50 μg/ml, respectively. Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance; Zcr, zeocin resistance.
Strain used for the LacZ reversion assay.