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. 2006 Jul;188(13):4787–4800. doi: 10.1128/JB.00066-06

FIG. 2.

FIG. 2.

Expression of proteins encoded by the cag pathogenicity island. H. pylori strain 26695 Sc#7 (WT) and an isogenic cag PAI deletion mutant strain (ΔcagPAI) were lysed, and the proteomes of these strains were analyzed by 2D-DIGE/MS, as described in Materials and Methods. Seven H. pylori Cag proteins (Cag3, VirB11/ATPase, CagZ/6, CagX/8, CagS/13, CagM/16, and CagA/26) (Table 2) were expressed by the wild-type strain but not by the mutant strain. (A) Representative data from a 2D-DIGE experiment using a gel with pH range of 4 to 7. CagZ/6 and VirB11/ATPase are indicated. VirB11/ATPase was detected in two isoforms (panel 2). (B) Representative data from a 2D-DIGE experiment using a gel with a pH range of 7 to 11. Cag3, CagX/8, CagM/16, CagS/13, and CagA/26 are indicated. Cag3 was detected in two isoforms (panel 1), and CagA/26 was detected in multiple isoforms (panel 3).