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. 2006 Jul;188(13):4787–4800. doi: 10.1128/JB.00066-06

FIG. 4.

FIG. 4.

Protein-protein interactions among H. pylori Cag proteins. Yeast cells were cotransformed with plasmids encoding the indicated fusion proteins. Cotransformed yeast cells (normalized based on optical density) were 10-fold serially diluted. Identical inocula were plated onto two types of media (SD medium lacking tryptophan and leucine and SD medium lacking tryptophan, leucine, and histidine). All of the cotransformed yeast cells grew on SD plates containing 2% glucose and lacking tryptophan and leucine. Growth of the cotransformed yeast cells on SD medium lacking tryptophan, leucine, and histidine provided evidence for the occurrence of protein-protein interactions. Panel A depicts three of the four homotypic interactions identified in the current study. Cag3(A) is a full-length protein, and Cag3(B) is a gene fragment lacking the predicted signal sequence. Panel B shows representative heterotypic interactions. GAL4AD-WT plus GAL4BD-WT are a positive control, and GAL4AD-WT plus GAL4BD-pLC are a negative control.