TABLE 3.
H. pylori genes selected for analysis using a yeast two-hybrid system
| H. pylori 26695 gene | Protein | Agrobacterium homologue | Identified by 2D-DIGEa | Necessary for CagAp-Tyra,b | Necessary for IL-8 secretiona,b |
|---|---|---|---|---|---|
| HP0522 | Cag3 | + | + | + | |
| HP0524 | Cag5 | VirD4 | + | − | |
| HP0525 | VirB11/ATPase | VirB11 | + | + | + |
| HP0526 | CagZ/6 | + | − | − | |
| HP0527 | CagY/7 | VirB10 | + | + | |
| HP0528 | CagX/8 | VirB9 | + | + | + |
| HP0530 | CagV/10 | VirB8 | + | + | |
| HP0532 | CagT/12 | VirB7 | + | + | |
| HP0534 | CagS/13 | + | − | − | |
| HP0537 | CagM/16 | + | + | + | |
| HP0540 | CagI/19 | + | − | ||
| HP0542 | CagG/21 | + | − | ||
| HP0543 | CagF/22 | + | − | ||
| HP0544 | CagE/23/PicB | VirB4 | + | + |
a
+, yes; −, no.
b
Based on analysis of interactions between H. pylori strain 26695 and AGS cells (27).