TABLE 2.

Real-time RT-PCR analysis of SapB and chaplin gene expression in surfactin-treated S. coelicolor

Gene	Untreateda	Surfactin treatedb	Relative changec (fold)
ramC	0.33 ± 0.03d	4.87 ± 3.23	Up ∼15
chpA	0.51 ± 0.22	0.01 ± 0.01	Down >50
chpH	0.88 ± 0.10	1.75 ± 0.55	Up ∼2
rpoB	0.69 ± 0.07	0.69 ± 0.07	None

^aRNA extracted from mycelia outside the bald-halo zone.

^bRNA extracted from mycelia within bald halo.

^cChange in mRNA abundance from surfactin-treated samples relative to untreated control.

^dValues are average relative nanograms of mRNA, normalized to rpoB, ± the standard deviation from triplicate samples. Value for rpoB is from combined untreated and surfactin-treated cultures.