FIG. 1.
Sequences of recombination substrates for TndX. (A) Comparison of the sequences of attTn formed within the circular joined intermediate of Tn5397 and the preferred target site in C. difficile, attBCd. (B) Sequences of the known target sites for Tn5397 in C. difficile (attBCd) and B. subtilis (attBs2, attBs3, attBs4, and attBs5a). Black shading indicates identity, dark-gray shading indicates 80% conservation, and light-gray shading indicates 60% conservation. The two asterisks above the sequences 5′ GA indicate the dinucleotides that are inferred to take part in the DNA cleavage and rejoining.