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. 2006 Jul;188(13):4983–4991. doi: 10.1128/JB.00170-06

FIG. 3.

FIG. 3.

Analysis of msp1β transcripts by Southern blotting with (+RT) and without (−RT) reverse transcription. A. marginale strain St. Maries RT-PCR products were hybridized with a DIG-labeled msp1β probe. cDNA samples collected from calf C942b1 during acute rickettsemia (A) and from calf C956bl presplenectomy (A) and postsplenectomy (B) were amplified with primers specific for each msp1β transcript (Table 1). A. marginale genomic DNA was used as a positive control (B). Lanes 1 to 5 represent the primer sets used: 1, msp1β1; 2, msp1β2; 3, msp1βpg3; 4, msp1βpg2; 5, msp1βpg1. For the A. marginale genomic DNA (B), the image in lane 5 is the result of a longer exposure of the same gel compared to the images of lanes 1 to 4. The sizes of digoxigenin-labeled markers (lane M) are indicated in base pairs on the right of the same gel as shown.