. 2006 Jul;188(13):4983–4991. doi: 10.1128/JB.00170-06

TABLE 1.

Primers used for PCR amplification of msp1β genes

Primer^a	Primer sequence (5′-3′)	Product amplified	Product size (bp)
Msp1b1 TF	CTTGACCAGAGCATTGACGCAC	msp1β1 transcript	1,875
Msp1b1/pg3 TR	AGAAGACTGCTGTTGTTGCTGC	Probe
Msp1b2 TF	GTAACGAGCTTGCACAAATATGTGGG	msp1β2 transcript	695
Msp1b2 TR	GGAAGAAGACTGCTGTTGTGCAG	msp1β2 transcript
Msp1bpg3 TF	GTCTATTGGCGATGCATTTGGCG	msp1β_pg3 transcript	979
Msp1b1/pg3 TR	AGAAGACTGCTGTTGTTGCTGC	msp1β_pg3 transcript
Msp1bpg2 TF	CCCACCTTGTGTGCATGGC	msp1β_pg2 transcript	1,182
Msp1bpg2 TR	CGCCAAAGTACACCAAAGGCC	msp1β_pg2 transcript
Msp1bpg1 TF	CAGAATTAGAGTATATCGCCCTGTATGCATG	msp1β_pg1 transcript	536
Msp1bpg1 TR	CCCAGTACGGGGGTTTCCC	msp1β_pg1 transcript
Msp1b1/b2 OF	CGGTTATCAAGACATTGTTAAGTAGGTAGGTG	msp1β1 ORF	2,530
Msp1b1 OR	GCCGAATATGCGCAGATGGC	msp1β1 ORF
Msp1b1/b2 OF	CGGTTATCAAGACATTGTTAAGTAGGTAGGTG	msp1β2 ORF	2,338
Msp1b2 OR	CTACCACGTTCTTGTATCCACACAAGG	msp1β2 ORF
Msp1bpg3 OF	GCATAGGGAAGATTGAAGTACCAGC	msp1β_pg3 ORF	2,096
Msp1bpg3 OR	CTACACCGTGTCAGTTAAAGGTAGGG	msp1β_pg3 ORF
Msp1bpg2 OF	CACAGCAGCATATTCAGGAATGTTGAAG	msp1β_pg2 ORF	1,466
Msp1bpg2 OR	GCGATTTTCGCCTCACAGAGC	msp1β_pg2 ORF
Msp1bpg1 OF	CATCATCTCGCAGATGAGCACTCG	msp1β_pg1 ORF	827
Msp1bpg1 OR	GCGATACAACAGCCACAAAGCG	msp1β_pg1 ORF
Msp1b1/b2 EF	ATGACAGAAGACGACAAGCAACAAC	Probe

TF and TR indicate forward and reverse primers designed to amplify specific msp1β transcripts. OF and OR indicate forward and reverse primers designed for the amplification of each msp1β open reading frame (ORF). EF indicates a forward primer designed for expression of MSP161 and MSP162.