FIG. 5.

fimA is transcribed from a PilR- and σ⁵⁴-dependent promoter. Primer extension reactions were performed on 50 μg of RNA from wild-type, pilR1, and rpoN1 strains using a primer 42 bp downstream of the translational start site. Shown is the σ⁵⁴ promoter region with the −12 and −24 elements indicated along with the transcriptional start point. Product sizes were determined by comparison to a sequencing ladder generated using the same primer (lanes A, C, G, and T).