Electron micrographs of P. aeruginosa Flp pili. (A to C and E to G) TEM views at magnifications of ×50,000 (A and B), ×80,000 (C), or ×140,000 (E to G) after immunogold labeling of Flp-producing PAO1 strains (PAO1/pMMBflp). In panel E, a nonlabeled flagellar structure is clearly visible next to a labeled Flp fiber with bundled filaments (see arrows). (D) Fourier transformation and ×4 enlargement of a small region of the labeled Flp filament.